Strain Collection
We are in the process of creating a web-based searchable form for identifying strains in our collection that meet your criteria. In the meantime, you can find below the database in Excel format, containing all strains in the collection and the most common metadata requested by clients (year of isolation, location and species of isolation, presence of virulence genes, etc). The second tab in this file contains an explanation of column headers. Note the first two numbers of an ECRC isolate name indicates the year we received that isolate (ie. strain 12.1234 was the 1234th isolate archived in 2012). If you are looking for other information, please contact Ed Dudley."
Isolates Database in Excel format
Sequenced Isolates
Through internal projects and external collaborations, most recently through involvement with the FDA's GenomeTrakr network, we have genome sequences for over 5,000 isolates, and anticipate growing this to over 6,000 by the end of 2021. Nearly all of our sequenced isolates, plus identified virulence, stress, and antimicrobial resistance genes, can be found in the NCBI Pathogen Detection database.
Ordering E.coli Strains
You may either select strains on your own, or contact Ed Dudley for assistance. Please send strain requests to ECRC@psu.edu, along with information about characteristics you are looking for (certain serogroups, presence of virulence genes, etc). Also, please send the name and contact information for shipping, as well as this information for individual(s) in your organization who have authority to sign a Materials Transfer Agreement (MTA). The process that follows:
- We will grow these isolates and confirm any phenotypes/genotypes that you require. We will contact you if any isolates fail our screens;
- We send your request to Penn State's Office of Sponsored Programs (OSP), who will contact your organization to sign the MTA;
- iAfter returning the MTA, Penn State will countersign, and send the final document to us, authorizing us to ship the strains;
- If your organization is outside the United States, additional procedures may be needed depending on the virulence potential of the isolates you request.
Our charge is $100 or $160/isolate for non-profit and for profit groups, respectively. Please note these prices do not include shipping, and this price includes all tests we perform to verify phenotype/genotype prior to sending. If you have a large number of characteristics you need verified, please contact us to discuss your project further. If your request seems standard, such as requiring isolates encoding particular Shiga toxins, please contact Ed Dudley first as we likely have isolates we can recommend.