Edward G Dudley, Ph.D.

Edward G Dudley, Ph.D.

  • Director of E. coli Reference Center
  • Professor of Food Science
427 Rodney A. Erickson Food Science Building
University Park, PA 16802

Areas of Expertise

  • Microbiology
  • Microbial pathogens
  • Molecular microbiology
  • Microbial genomics
  • Food Safety
  • Food Microbiology

Websites

Education

  • Postdoctoral research, University of Maryland School of Medicine
  • Ph.D., University of Wisconsin Madison, Bacteriology, 2000
  • M.S., University of Wisconsin Madison, Food Science, 1994
  • B.S., Penn State University, Microbiology, 1991

Other Affiliations

Molecular Cellular and Integrative Biosciences

Center for Molecular Immunology and Infectious Disease

Center for Infectious Disease Dynamics

Pathobiology Graduate Program

Research Interests

My program uses molecular biology, biochemistry, and molecular biology techniques to better understand the biology and evolution of foodborne pathogens, and to develop improved methods of tracking the spread of these organisms from farm-to-fork.

Escherichia coli O157:H7 is a foodborne pathogen that causes illness through mechanisms that include the elaboration of an AB5 protein called Shiga toxin. When I joined Penn State in 2007, the research community was just beginning to use genomic techniques to define the phylogeny of global isolates and to explain the heterogeneity observed in terms of virulence potential and toxin production. I took this opportunity to develop a program investigating the factors driving differences in toxin production using whole genomic analysis (mainly in collaboration with Dr. Mark Eppinger at the University of Texas-San Antonio), investigation of polymorphisms encoded within bacteriophage that carry the Shiga toxin genes, and interactions with members of the gut microflora. Most notably, we have found that certain strains categorized as low toxin producers synthesize high levels of toxin when co-cultured with other commensal strains of E. coli. We have also shown that inoculation of mice with such co-cultures causes more serious disease than inoculation with the pathogen alone. Future work is directed at better understanding the mechanisms behind these observations.

We are proudly a member of the US Food and Drug Administration's GenomeTrakr program, representing the Commonwealth of Pennsylvania. This program is a collaboration between Penn State and the PA Department of Health. Our goal is to help populate the whole genome sequence databases with foodborne isolates, particularly E. coli and Salmonella from food and environmental sources, and to help organizations such as our Department of Health and the food industry to gain the knowledge needed to enter this field or to gain the knowledge necessary to benefit from advances in genomic sciences.

In 2007, several high profile papers described the function of elements first discovered in bacterial genomes 30 years earlier, called CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). These manuscripts and those that followed elegantly described how CRISPR is an adaptive immune system for prokaryotes designed to protect cells against foreign DNA including bacteriophage and plasmids. We were some of the first to recognize that these rapidly evolving elements could be used as molecular signatures for strain identification during foodborne outbreaks. We have studied CRISPR diversity primarily in Salmonella enterica, with additional work in Shiga toxin producing Escherichia coli. We have demonstrated their utility in molecular subtyping, and for the development of rapid methods of pathogen identification. More recent (unpublished) work has begun to elucidate the function of CRISPR in S. enterica, as it becomes more apparent that CRISPR has functions outside of those of immunity. 

Research Information

Table S5.4: Rare codon tRNA usage in E. coli O157:H7 Sakai
Table S5.5: Tandem rare codons in the E. coli O157:H7 Sakai genome

Keefer, A. B.*, Xiaoli, L.*, M'ikanatha, N. M., Yao, K., Hoffmann, M., and E. G. Dudley. 2018. Retrospective whole-genome sequencing analysis distinguished PFGE and drug resistance matched retail meat and clinical Salmonella isolates. bioRxiv 356857.

Abelman, R. L., N. M. M'ikanatha, and E. G. Dudley. 2018. Whole genome sequencing of antimicrobial-resistant Shigella sonnei associated with infection acquired from international and domestic setting reveals gene allelic variants that predict global lineages. bioRxiv 361196.

Xiaoli L, H. M. Figler, K. Goswami Banerjee, C. S. Hayes, and E. G. Dudley. 2018. Non-pathogenic Escherichia coli Enhance Stx2a Production of E. coli O157:H7 Through Both bamA-Dependent and Independent Mechanisms. Front Microbiol. 9:1325.

Zhang H, E. G. Dudley, and F. Harte. 2017, Critical synergistic concentration of lecithin phospholipids improves the antimicrobial activity of eugenol against Escherichia coli. Appl Environ Microbiol. 83: e01583-17.

Yang X, E. Huang, M. Yesil, L. Xiaoli, E. G. Dudley, and A. E. Yousef AE. 2017. Draft genome sequence of Brevibacillus laterosporus OSY-I1, a strain that produces Brevibacillin, which combats drug-resistant Gram-positive bacteria. Genome Announc. 5: e01093-17.

Gerst M.M., E. G. Dudley, L. Xiaoli, and A. E. Yousef. 2017. Draft genome sequence of Bacillus velezensis GF610, a producer of potent anti-Listeria agents. Genome Announc. 5: e01046-17.

DiMarzio M, B. Rusconi, N. H. Yennawar, M. Eppinger, A. D. Patterson, and E. G. Dudley. 2017. Identification of a mouse Lactobacillus johnsonii strain with deconjugase activity against the FXR antagonist T-β-MCA. PLoS One. 12:e0183564

Zhang H, E. G. Dudley, P. M. Davidson, and F. Harte. 2017. Critical concentration of lecithin enhances the antimicrobial activity of eugenol against Escherichia coli. Appl Environ Microbiol. 83: e03467-16.

Scheinberg J.A., E. G. Dudley, J. Campbell, B. Roberts, M. DiMarzio, C. DebRoy, and C. N. Cutter. 2017. Prevalence and Phylogenetic Characterization of Escherichia coli and Hygiene Indicator Bacteria Isolated from Leafy Green Produce, Beef, and Pork Obtained from Farmers' Markets in Pennsylvania. J Food Prot. 80:237-244

Uhlich, G. A., G. C. Paoli, X. Zhang, E. G. Dudley, H. M. Figler, B. J. Cottrell, and E. Andreozzi. 2017. Whole-genome sequence of Escherichia coli serotype O157:H7 strain PA20. Genome Announc. 5:e01460-16.

Danagamage, T. N., P. Patterson, E. Wallner-Pendleton, D. Trampel, N. Shariat, E. G. Dudley, B. M. Jayarao, and S. Kariyawasam. 2016. Longitudinal monitoring of successive commercial layer flocks for Salmonella enteria serovar Enteritidis. 13:618-625

Very, K. J., M. K, Kirchner, N. Shariat, W. Cottrell, C. H. Sandt, E. G. Dudley, S. Kariyawasam, and B. M. Jayarao. 2016. Prevalence and spatial distribution of Salmonella infections in the Pennsylvania raccoon (Procyon lotor). Zoonoses Public Health 63:223-233.

Figler, H.M., and E. G. Dudley. 2016. The interplay of Escherichia coli O157:H7 and commensal E. coli: the importance of strain-level identification. Expert Rev. Gastroenterol Hepatol.10:415-417.

Valderrama, W. B., E. G. Dudley, S. Doores, and C. N. Cutter. 2016. Commercially available rapid methods for detection of selected foodborne pathogens. Crit. Rev. Food Sci. Nutr. 56:1519-1531.

Barrangou, R., and E. G. Dudley. 2016. CRISPR-based typing and next-generation tracking technologies. Ann. Rev. Food Sci. Technol. 7:395-411.

Ivanov, Y. V., N. Shariat, K. B. Register, B. Linz, I. Rivera, K. Hu, E. G. Dudley, and E. T. Harvill. 2015. A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease. BMC Genomics 26:863.

Park, J., Y. Zhang, C. Chen, E. G. Dudley, and E. T. Harvill. 2015. Diversity of Secretion Systems Associated with Virulence Characteristics of the Classical Bordetellae. Microbiology 161:2328-2340

Yin, S., B. Rusconi, F. Sanjar, K. Goswami, L. Xiaoli, M. Eppinger, and E. G. Dudley. 2015. Escherichia coli O157:H7 strains harbor at least three distinct sequence types of Shiga toxin 2a-converting phages. BMC Genomics. 16:733.

Goswami, K., C. Chen, L. Xiaoli, K. A. Eaton, and E. G. Dudley. 2015. Co-culturing Escherichia coli O157:H7 with a non-pathogenic E. coli strain increases toxin production and virulence in a germ-free mouse model. Infect. Immun. 83:4185-4193.

Jiang, Y., S. Yin, E. G. Dudley, and C. N. Cutter. 2015. Diversity of CRISPR loci and virulence genes in pathogenic Escherichia coli isolates from various sources. Int. J. Food Microbiol. 204:41-46.

Shariat, N., R. E. Timme, J. B. Pettengill, R. Barrangou, and E. G. Dudley. 2015. Characterization and evolution of Salmonella CRISPR-cas systems. Microbiology UK. 161: 374-386.

Deng X, Shariat N, Driebe EM, Roe CC, Tolar B, Trees E, Keim P, Zhang W, Dudley EG, Fields PI, Engelthaler DM. 2015. Comparative analysis of subtyping methods against a whole-genome-sequencing standard for Salmonella enterica Serotype Enteritidis. J Clin Microbiol. 53:212-218.

Sanjar, J., T. H. Hazen, S. M. Shah, S. S. Koenig, S. Agrawal, S. Daugherty, L. Sadzewicz, L. J. Tallon, M. K. Mammel, P. Feng, R. Soderlund, P. I. Tarr, C. DebRoy, E. G. Dudley, T. A. Cebula, J. Ravel, C. M. Fraser, D. A. Rasko, and M. Eppinger. 2014. Genome sequence of Escherichia coli O157:H7 stain 2886-75, associated with the first reported case of human infection in the United States. Genome Announc. 2:e01120-13.

Shariat, N., C. H. Sandt, M. J. DiMarzio, R. Barrangou, and E. G. Dudley. 2013. CRISPR-MVLST subtyping of Salmonella enterica subsp. enterica serovars Typhimurium and Heidelberg and application in identifying outbreak isolates. BMC Microbiol. 13:254.

Shariat, N., and E. G. Dudley. 2013. CRISPRs: Molecular signatures used for pathogen subtyping. Appl. Environ. Microbiol. 80:430-439.

Shariat N., and E. G. Dudley. 2013. Where are we heading with Salmonella molecular subtyping? Future Microbiol. 8:1231–1233

Loquasto, J. R., R. Barrangou, E. G. Dudley, B. Stahl, C. Chen, and R. F. Roberts. Bifidobacterium animalis subsp. lactis ATCC 27673 is a genomically unique strain within this conserved subspecies. Appl. Environ. Microbiol. 79:6903-6910.

Yin, S., M. Jensen, J. Bai, C. DebRoy, R. Barrangou, and E. G. Dudley. Evolutionary Divergence of Shiga Toxin-producing Escherichia coli is Reflected in CRISPR Spacer Composition. Appl. Environ. Microbiol. 79:5910-5920.

Chen, C., C. A. Blumentritt, M. M. Curtis, V. Sperandio, A. G. Torres, and E. G. Dudley. 2013. Restrictive streptomycin-resistant mutations decrease the formation of attaching and effacing lesions in Escherichia coli O157:H7 strains. Antimicrob. Agents Chemother. 57:4260-4266.

DiMarzio, M., N. Shariat, S. Kariyawasam, R. Barrangou, and E. G. Dudley. 2013. Antibiotic resistance in Salmonella Typhimurium associates with CRISPR sequence type. Antimicrob. Agents Chemother. 57:4282-4289.

Uhlich, G. A., C. Y. Chen, B. J. Cottrell, C. S. Hofmann, E. G. Dudley, T. P. Strobaugh, and L. H. Nguyen. 2013. Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157:H7. Microbiology UK. 159:1586-1596.

Shariat, N., M. K. Kirchner, C. H. Sandt, E. Trees, R. Barrangou, and E. G. Dudley. 2013. CRISPR-MVLST Subtyping of Salmonella serovar Newport Outbreak Isolates and Determination of the Relationship Between CRISPR-MVLST and PFGE. J. Clin. Microbiol. 51:2328-2336.

Valderrama, W. B., E. G. Dudley, S. Doores, and C. N. Cutter. Commercially available rapid methods for detection of selected foodborne pathogens. Accepted in Crit. Rev. Fd. Sc. Nutr.

Svoboda, A. L., E. G. Dudley, C. DebRoy, E. W. Mills, and C. N. Cutter. Presence of Shiga toxin-producing Escherichia coli O-groups in small and very small beef processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay. Foodborne Pathog. Dis. 10:789-795.

Eppinger, M., S. Daugherty, S. Agrawai, K. Galens, N. Sengamalay, L. Sadzewicz, L. Tallon, T. A. Cebula, M. K. Mammel, P. Feng, R. Soderlund, P. I. Tarr, C. DebRoy, E. G. Dudley, C. M. Fraser, J. Ravel. 2013. Whole-genome draft sequences of 26 enterohemorrhagic Escherichia coli O157:H7 strains. Genom. Announc. 1:e00134-12.

Chen C., C. R. Lewis, K. Goswami, E. L. Roberts, C. Debroy, and E. G. Dudley. 2013. Identification and Characterization of Spontaneous Deletions within the Sp11-Sp12 Prophage Region of Escherichia coli O157:H7 Sakai. Appl. Environ. Microbiol. 79:1934-1941.

Shariat, N., M. J. DiMarzio, S. Yin, L. Dettinger, C. H. Sandt, J. R. Lute, R. Barrangou, and E. G. Dudley. 2013. The combination of CRISPR-MVLST and PFGE provides increased discriminatory power for differentiating human clinical isolates of Salmonella enterica subsp. enterica serovar Enteritidis. Food Microbiol. 34:164-173.

Weyrich L.S., O. Y. Rolin, S. J. Muse, J. Park, N. Spidale, M. J. Kennett, S. E. Hester, C Chen, E. G. Dudley, and E. T. Harvill. 2012. A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. PloS One 7:e45892

Verghese B., N. D. 3rd Schwalm, E. G. Dudley, and S. J. Knabel. 2012. A combined multi-virulence-locus sequence typing and Staphylococcal Cassette Chromosome mec typing scheme possesses enhanced discriminatory power for genotyping MRSA. Infect Genet Evol. 12:1816-1821.

Miller D.M., E. G. Dudley, and R. F. Roberts. 2012. Technical note: development of a quantitative PCR method for monitoring strain dynamics during yogurt manufacture. J. Dairy Sci. 95:4868-4872.

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Just a few of my favorite things

Publications

The Intriguing Interaction of Escherichia coli with the Host Environment and Innovative Strategies To Interfere with Colonization: a Summary of the 2019 E. coli and the Mucosal Immune System Meeting.
Applied and Environmental Microbiology, Cox, E, Aloulou, M, Fleckenstein, J, Schäffer, C, Sjöling, Å, Schüller, S, Hanevik, K, Devriendt, B, Zhang, W, Svennerholm, A, Dudley, Edward, 2020

Special Section on the Third International E. coli and the Mucosal Immune System (ECMIS) Symposium.
Applied and Environmental Microbiology, Dudley, Edward, 2020

Novel effective bacillus cereus group species "bacillus clarus" is represented by antibiotic-producing strain ATCC 21929 isolated from soil
mSphere, Méndez Acevedo, Marysabel, Carroll, Laura M., Mukherjee, Manjari, Mills, Emma, Xiaoli, Lingzi, Dudley, Edward G., Kovac, Jasna, 2020

Draft genome sequences of isolates of diverse host origin from the E. coli reference center at penn state university
Microbiology Resource Announcements, Lacher, David W., Mammel, Mark K., Gangiredla, Jayanthi, Gebru, Solomon T., Barnaba, Tammy J., Majowicz, Sydney A., Dudley, Edward G., 2020

Whole-genome sequences of salmonella isolates from an ecological wastewater treatment system
Microbiology Resource Announcements, Connolly, Charles J., Kaminsky, Laura, Pinto, Gabriella N., Sinclair, Priscilla C., Bajracharya, Gyasu, Yan, Runan, Nawrocki, Erin M., Dudley, Edward G., Kovaca, Jasna, 2020

A Putative Microcin Amplifies Shiga Toxin 2a Production of Escherichia coli O157:H7
Journal of bacteriology, Mosso, Hillary M., Xiaoli, Lingzi, Banerjee, Kakolie, Hoffmann, Maria, Yao, Kuan, Dudley, Edward G., 2019

Detection of CTX-M-1 extended-spectrum beta-lactamase among ceftiofur-resistant Salmonella enterica clinical isolates of poultry
Journal of Veterinary Diagnostic Investigation, Denagamage, Thomas N., Wallner-Pendleton, Eva A., Jayarao, Bhushan M., Xiaoli, Lingzi, Dudley, Edward G., Wolfgang, David, Kariyawasam, Subhashinie, 2019

Use of whole genome sequencing in surveillance for antimicrobial-resistant Shigella sonnei infections acquired from domestic and international sources
Microbial genomics, Abelman, Rebecca L., M'ikanatha, Nkuchia M., Figler, Hillary M., Dudley, Edward G., 2019

Retrospective whole-genome sequencing analysis distinguished PFGE and drug-resistance-matched retail meat and clinical Salmonella isolates
Microbiology (United Kingdom), Keefer, Andrea B., Xiaoli, Lingzi, M’ikanatha, Nkuchia M., Yao, Kuan, Hoffmann, Maria, Dudley, Edward G., 2019

Whole-genome sequences of Escherichia coli isolates from cocoa beans imported from bolivia
Microbiology Resource Announcements, Nwanosike, Hephzibah, Chung, Taejung, Xiaoli, Lingzi, Condello, Megan, Dudley, Edward G., Kovac, Jasna, 2019

Whole-genome sequencing reveals resistome of highly drug-resistant retail meat and human Salmonella Dublin
Zoonoses and Public Health, Eyler, Andrea B., M'ikanatha, Nkuchia M., Xiaoli, Lingzi, Dudley, Edward G., 2019

Non-pathogenic escherichia coli enhance Stx2a production of E. coli O157:H7 through both bamA-dependent and independent mechanisms
Frontiers in Microbiology, Xiaoli, Lingzi, Figler, Hillary M., Banerjee, Kakolie Goswami, Hayes, Christopher S., Dudley, Edward G., 2018

Critical synergistic concentration of lecithin phospholipids improves the antimicrobial activity of eugenol against Escherichia coli
Applied and environmental microbiology, Zhang, Haoshu, Dudley, Edward G., Harte, Federico, 2017

Identification of a mouse Lactobacillus johnsonii strain with deconjugase activity against the FXR antagonist T-β-MCA
PLoS One, DiMarzio, Michael, Rusconi, Brigida, Yennawar, Neela, Eppinger, Mark, Patterson, Andrew David, Dudley, Edward G., 2017

Critical concentration of lecithin enhances the antimicrobial activity of eugenol against Escherichia coli
Applied and environmental microbiology, Zhang, Haoshu, Dudley, Edward G., Davidson, P. Michael, Harte, Federico, 2017

Prevalence and phylogenetic characterization of Escherichia coli and hygiene indicator bacteria isolated from leafy green produce, beef, and pork obtained from farmers' markets in Pennsylvania
Journal of Food Protection, Scheinberg, Joshua A., Dudley, Edward G., Campbell, Jonathan Alexander, Roberts, Beth, DiMarzio, Michael, Debroy, Chitrita, Cutter, Catherine Nettles, 2017

Whole-genome sequence of Escherichia coli serotype O157:H7 strain PA20
Genome Announcements, Uhlich, Gaylen A., Paoli, George C., Zhang, Xinmin, Dudley, Edward G., Figler, Hillary M., Cottrell, Bryan J., Andreozzi, Elisa, 2017

Draft genome sequence of Bacillus velezensis GF610, a producer of potent anti- Listeria agents
Genome Announcements, Gerst, Michelle M., Dudley, Edward G., Xiaoli, Lingzi, Yousef, Ahmed E., 2017

Draft genome sequence of Brevibacillus laterosporus OSY-I1, a strain that produces brevibacillin, which combats drug-resistant Gram-positive bacteria
Genome Announcements, Yang, Xu, Huang, En, Yesil, Mustafa, Xiaoli, Lingzi, Dudley, Edward G., Yousef, Ahmed E., 2017

Longitudinal monitoring of successive commercial layer flocks for salmonella enterica serovar enteritidis
Foodborne pathogens and disease, Denagamage, Thomas N., Patterson, Paul, Wallner-Pendleton, Eva, Trampel, Darrell, Shariat, Nikki, Dudley, Edward G., Jayarao, Bhushan M., Kariyawasam, Subhashinie, 2016

Prevalence and Spatial Distribution of Salmonella Infections in the Pennsylvania Raccoon (Procyon lotor)
Zoonoses and Public Health, Very, K. J., Kirchner, M. K., Shariat, N., Cottrell, W., Sandt, C. H., Dudley, Edward G., Kariyawasam, S., Jayarao, Bhushan M., 2016

Commercially available rapid methods for detection of selected foodborne pathogens
Critical Reviews in Food Science and Nutrition, Valderrama, W, Dudley, Edward, Doores, S, Cutter, C, 2016

CRISPR-based typing and next-generation tracking technologies
Annual Reviews of Food Science and Technology, Dudley, Edward, Barrangou, Rodolphe, 2016

The interplay of Escherichia coli O157:H7 and commensal E. coli: the importance of strain-level identification.
Expert Reviews in Gastroenterology and Hepatology, Figler, Hillary, Dudley, Edward, 2016

Diversity of secretion systems associated with virulence characteristics of the classical bordetellae
Microbiology (United Kingdom), Park, Jihye, Zhang, Ying, Chen, Chun, Dudley, Edward G., Harvill, Eric T., 2015

A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease
BMC genomics, Ivanov, Yury V., Shariat, Nikki, Register, Karen B., Linz, Bodo, Rivera, Israel, Hu, Kai, Dudley, Edward G., Harvill, Eric Thomas, 2015

Escherichia coli O157
BMC genomics, Yin, Shuang, Rusconi, Brigida, Sanjar, Fatemeh, Goswami, Kakolie, Xiaoli, Lingzi, Eppinger, Mark, Dudley, Edward G., 2015

Diversity of CRISPR loci and virulence genes in pathogenic Escherichia coli isolates from various sources
International journal of food microbiology, Jiang, Yun, Yin, Shuang, Dudley, Edward G., Cutter, Catherine N., 2015

Comparative analysis of subtyping methods against a whole-genome-sequencing standard for Salmonella enterica serotype Enteritidis
Journal of clinical microbiology, Deng, Xiangyu, Shariat, Nikki, Driebe, Elizabeth M., Roe, Chandler C., Tolar, Beth, Trees, Eija, Keim, Paul, Zhang, Wei, Dudley, Edward G., Fields, Patricia I., Engelthaler, David M., 2015

Characterization and evolution of Salmonella CRISPR-Cas systems
Microbiology (United Kingdom), Shariat, Nikki, Timme, Ruth E., Pettengill, James B., Barrangou, Rodolphe, Dudley, Edward G., 2015

Coculture of Escherichia coli O157:H7 with a nonpathogenic E. coli strain increases toxin production and virulence in a germfree mouse model
Infection and Immunity, Goswami, Kakolie, Chen, Chun, Xiaoli, Lingzi, Eaton, Kathryn A., Dudley, Edward G., 2015

Complete nucleotide sequence of pRS218, a large virulent plasmid that augment pathogenic potential of meningitis-associated Escherichia coli strain RS218
BMC Microbiology, Wijetunge, D, Karunathilake, E, Chaudhari, A, Katani, R, Dudley, Edward, Kapur, V, DebRoy, C, Kariyawasam, S, 2015

Complete nucleotide sequence of pRS218, a large virulence plasmid, that augments pathogenic potential of meningitis-associated Escherichia coli strain RS218
BMC microbiology, Wijetunge, Dona Saumya S., Karunathilake, Kurundu Hewage Eranda M., Chaudhari, Atul, Katani, Robab, Dudley, Edward G., Kapur, Vivek, Debroy, Chitrita, Kariyawasam, Subhashinie, 2014

Genome sequence of Escherichia coli O157
Genome Announcements, Sanjar, Fatemeh, Hazen, Tracy H., Shah, Sadiq M., Koenig, Sara S.K., Agrawal, Sonia, Daugherty, Sean, Sadzewicz, Lisa, Tallon, Luke J., Mammel, Mark K., Feng, Peter, Soderlund, Robert, Tarr, Phillip I., DebRoy, Chitrita, Dudley, Edward G., Cebula, Thomas A., Ravel, Jacques, Fraser, Claire M., Rasko, David A., Eppinger, Mark, 2014

CRISPRs: Molecular signatures used for pathogen subtyping
Applied and Environmental Microbiology, Shariat, N, Dudley, Edward, 2014

CRISPRs: molecular markers for tracking antibiotic resistant strains of Salmonella enterica
Winter 2014 newsletter of the Alliance for the Prudent Use of Antibiotics, Dudley, Edward, 2014

Ceramide trihexoside bottom spot as a receptor to detect Shiga toxin 2 from STEC
August 2014 newsletter of Matreya, LLC, Xiaoli, L, Goswami, K, Dudley, Edward, 2014

CRISPR-MVLST subtyping of Salmonella enterica subsp. enterica serovars Typhimurium and Heidelberg and application in identifying outbreak isolates
BMC microbiology, Shariat, Nikki, Sandt, Carol H., Dimarzio, Michael J., Barrangou, Rodolphe, Dudley, Edward G., 2013

Bifidobacterium animalis subsp. lactis ATCC 27673 Is a genomically unique strain within its conserved subspecies
Applied and environmental microbiology, Loquasto, Joseph R., Barrangou, Rodolphe, Dudley, Edward G., Stahl, Buffy, Chen, Chun, Roberts, Robert F., 2013

The evolutionary divergence of shiga toxin-producing escherichia coli is reflected in clustered regularly interspaced short palindromic repeat (CRISPR) spacer composition
Applied and environmental microbiology, Yin, Shuang, Jensen, Mark A., Bai, Jiawei, DebRoy, Chitrita, Barrangou, Rodolphe, Dudley, Edward G., 2013

Presence of shiga toxin-producing escherichia coli o-groups in small and very-small beef-processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay
Foodborne pathogens and disease, Svoboda, Amanda L., Dudley, Edward G., Debroy, Chitrita, Mills, Edward W., Cutter, Catherine N., 2013

Restrictive streptomycin resistance mutations decrease the formation of attaching and effacing lesions in escherichia coli O157:H7 strains
Antimicrobial agents and chemotherapy, Chen, Chun, Blumentritt, Carla A., Curtis, Meredith M., Sperandio, Vanessa, Torres, Alfredo G., Dudley, Edward G., 2013

Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157
Microbiology (United Kingdom), Uhlich, Gaylen A., Chen, Chin Yi, Cottrell, Bryan J., Hofmann, Christopher S., Dudley, Edward G., Strobaugh, Terence P., Nguyen, Ly Huong, 2013

Subtyping of Salmonella enterica serovar newport outbreak isolates by CRISPR-MVLST and determination of the relationship between CRISPR-MVLST and PFGE results
Journal of clinical microbiology, Shariat, Nikki, Kirchner, Margaret K., Sandt, Carol H., Trees, Eija, Barrangou, Rodolphe, Dudley, Edward G., 2013

The combination of CRISPR-MVLST and PFGE provides increased discriminatory power for differentiating human clinical isolates of Salmonella enterica subsp. enterica serovar Enteritidis
Food Microbiology, Shariat, Nikki, DiMarzio, Michael J., Yin, Shuang, Dettinger, Lisa, Sandt, Carol H., Lute, James R., Barrangou, Rodolphe, Dudley, Edward G., 2013

Identification and characterization of spontaneous deletions within the Sp11-Sp12 prophage region of Escherichia coli O157:H7 Sakai
Applied and environmental microbiology, Chen, Chun, Lewis, Carrie R., Goswami, Kakolie, Roberts, Elisabeth L., DebRoy, Chitrita, Dudley, Edward G., 2013

Whole-genome draft sequences of 26 enterohemorrhagic Escherichia coli O157:H7 strains
Genome Announcements, Eppinger, Mark, Daugherty, Sean, Agrawal, Sonia, Galens, Kevin, Sengamalay, Naomi, Sadzewicz, Lisa, Tallon, Luke, Cebula, Thomas A., Mammel, Mark K., Feng, Peter, Soderlund, Robert, Tarr, Phillip I., DebRoy, Chitrita, Dudley, Edward G., Fraser, Claire M., Ravel, Jacques, 2013

Antibiotic resistance in Salmonella Typhimurium associates with CRISPR sequence type
Antimicrobial Agents and Chemotherapy, DiMarzio, M, Shariat, N, Kariyawasam, S, Barrangou, R, Dudley, Edward, 2013

Where are we heading with Salmonella molecular subtyping?
Future Microbiolology, Shariat, N, Dudley, Edward, 2013

A combined multi-virulence-locus sequence typing and Staphylococcal Cassette Chromosome mec typing scheme possesses enhanced discriminatory power for genotyping MRSA
Infection, Genetics and Evolution, Verghese, Bindhu, Schwalm, Nathan D., Dudley, Edward G., Knabel, Stephen John, 2012

Technical note
Journal of dairy science, Miller, D. M., Dudley, Edward G., Roberts, Robert F., 2012

Development of a qPCR method for evaluation of strain dynamics during yogurt fermentation
, Miller, D., Dudley, Edward, Roberts, Robert, 2012

Short communication: The complete genome sequence of Bifidobacterium animalis subspecies animalis ATCC 25527 T and comparative analysis of growth in milk with B. animalis subspecies lactis DSM 10140 T
Journal of dairy science, Loquasto, J. R., Barrangou, R., Dudley, E. G., Roberts, R. F., 2011

Escherichia coli O157:H7 of genotype lineage-specific polymorphism assay 211111 and clade 8 are common clinical isolates within pennsylvania
Foodborne pathogens and disease, Hartzell, Annette, Chen, Chun, Lewis, Carrie, Liu, Kuanqing, Reynolds, Stanley, Dudley, Edward G., 2011

Detection of shiga toxin-producing escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster
Foodborne pathogens and disease, Debroy, Chitrita, Roberts, Elisabeth, Valadez, Angela M., Dudley, Edward G., Cutter, Catherine N., 2011

Novel virulence gene and clustered regularly interspaced short palindromic repeat (CRISPR) multilocus sequence typing scheme for subtyping of the major serovars of Salmonella enterica subsp. enterica
Applied and Environmental Microbiology, Liu, Fenyun, Barrangou, Rodolphe, Gerner-Smidt, Peter, Ribot, Efrain M., Knabel, Stephen John, Dudley, Edward G., 2011

Multiplex PCR detection of shiga toxin-producing escherichia coli strains belonging to serogroups o157, o103, o91, o113, o145, o111, and o26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef
Journal of Food Protection, Valadez, Angela M., Debroy, Chitrita, Dudley, Edward G., Cutter, Catherine Nettles, 2011

Subtyping Salmonella serovar Enteritidis isolates from different sources using multilocus sequence typing based on virulence genes and CRISPRs
Applied and Environmental Microbiology, Liu, F, Kariyawasam, S, Jayarao, B, Barrangou, R, Gerner-Smidt, P, Ribot, E, Knabel, S, Dudley, Edward, 2011